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Method of detecting DNA by DNA hybridization method with the use of fluorescent resonance energy tra 发明授权

2022-07-31 3390 659K 0

专利信息

申请日期 2024-11-18 申请号 US10181826
公开(公告)号 US6830889B1 公开(公告)日 2004-12-14
公开国别 US 申请人省市代码 全国
申请人 Japan Science and Technology Agency
简介 A practically usable DNA detection method by a DNA hybridization method with the use of fluorescent resonance energy transfer wherein the background luminescence based on the luminescence of a donor and the direct excitation of an acceptor) affecting the sensitized luminescence of the acceptor is minimized so that a target DNA strand can be conveniently and very easily detected in a homogeneous solution. In this DNA detection method, a DNA is detected by the DNA hybridization method by using, as detection reagents, streptoavidin labeled with a fluorescent rare earth metal complex, a nucleic acid probe modified with biotin and a nucleic acid probe labeled with an organic cyanine pigment and taking advantage of florescent resonance energy transfer.


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