| 简介 |
A method of preparing a library of replenishable synthetic nucleic acid fragment pools is provided. Each of the pools represents one clone of a tiling set of genomic clones that provide substantially overlapping coverage of an entire genome, or a portion thereof. The fragment pools generated by this method can be replenished at any time without the need to re-isolate the original genomic clones that were used to create the tiling set. The pools can be used, for example, as a source of probes for applications such as nucleic acid hybridization or FISH. The libraries can be used, for example, to prepare nucleic acid arrays that can span an entire genome, or a portion thereof. |
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